The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids
- 1 January 1997
- journal article
- Published by Oxford University Press (OUP) in Glycobiology
- Vol. 7 (6) , 731-735
- https://doi.org/10.1093/glycob/7.6.731
Abstract
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monodonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T.cruzi was made.Keywords
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