Analysis of Lectin-Bound Glycoproteins in Snake Venom from the Elapidae and Viperidae Families
- 13 January 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 3 (3) , 383-392
- https://doi.org/10.1021/pr034052+
Abstract
This paper describes an efficient method of studying the glycoproteins found in snake venom. The glycosylation profiles of the Elapidae and Viperidae snake families were analyzed using FITC-labeled lectin glycoconjugates. The Con A-agarose affinity enrichment technique was used to fractionate glycoproteins from the N. naja kaouthia venom. The results revealed a large number of Con A binding glycoproteins, most of which have moderate to high molecular weights. To identify the proteins, the isolated glycoprotein fractions were subjected to two-dimensional electrophoresis and MALDI-TOF MS. Protein sequences were compared with published protein databases to determine for their biological functions. Keywords: snake venom • proteomics • lectin • glycoproteinKeywords
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