Presence of Conjugated 5‐Hydroxytryptamine in In Vivo Superfusates of Rat Spinal Cord

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Abstract
The presence of an acid‐hydrolyzable conjugate of 5‐hydroxytryptamine, presumably 5‐hydroxytryptamine‐O‐sulfate, was demonstrated in in vivo superfusates of rat spinal cord by HPLC with electrochemical detection. In untreated rats, the concentration of the 5‐hydroxytryptamine conjugate measured during the basal efflux of 5‐hydroxytryptamine did not differ from that measured during the release of 5‐hydroxytryptamine evoked by dl‐ρ‐chloroamphetamine. Pretreatment of the rats with clorgyline, an inhibitor of the enzyme monoamine oxidase (EC 1.4.3.4), or with probenecid did not alter the concentrations of conjugated 5‐hydroxytryptamine measured during the basal efflux of 5‐hydroxytryptamine, but did elevate the concentrations of conjugate measured during the evoked release of 5‐hydroxytryptamine.