Capillary gas chromatography/negative ion chemical ionization mass spectrometry for the quantification of bile acids including 1β-hydroxylated and unsaturated bile acids in serum and urine

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Abstract
12 bile acids, including 1 β‐hydroxylated and unsaturated bile acids, have been quantified by capillary gas chromatography/negative ion chemical ionization mass spectrometry, using the trimethylsilyl(TMS) ether derivatives of bile acid pentafluorobenzyl(PFB) esters. The analysis time is 12 min and the minimum measurable amount is 100 fg for each bile acid. Bile acids in 200 μL of serum and 50 μL of urine from healthy human adults were measured. These small sample sizes enhance the practicality of using this method as a screening test for bile acids in the serum and urine of human infants, where small sample size is a major problem.