Steroid-1-dehydrogenases in nocardioform bacteria studied by electrophoresis and immuno blotting techniques
- 1 January 1990
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 30 (6) , 415-423
- https://doi.org/10.1002/jobm.3620300608
Abstract
Fifteen nocardioform bacteria strains, capable of transforming steroid compounds were investigated with regard to their range of inducible steroid-1-dehydrogenase (St1DH)1 activities. The St1DHs of these bacteria were compared due to their immuno reactivity in Western blot experiments with a rabbit antiserum raised against the purified St1DH of Rhodococcus rhodochrous 7030. Four strains exhibited a strong immuno reactivity, irrespective of differences in the electrophoretic mobility of the enzymes. Fivestrains revealed significantly diminished reactivities, and in five strains with a very low St1DH content, no reactivity was found. One strain, designated as Nocardiaspec. 7151, exhibited a high, inducible St1DH activity, but no immuno reaction was found. The absence of immuno reactivity is discussed in connection with the considerably diminished electrophoretic mobility of this enzyme.This publication has 25 references indexed in Scilit:
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