Collagenolytic activity of pigmented pseudomonads
- 1 August 1970
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 16 (8) , 709-712
- https://doi.org/10.1139/m70-121
Abstract
A study of the collagenolytic activity of two strains of pigmented Pseudomonas showed that both organisms rapidly produced ninhydrin-positive materials from soluble collagen at 20 °C after a lag period of 2–6 days. When cells previously adapted to utilizing collagen were used as the inoculum, the lag period was eliminated, indicating that the enzyme(s) involved is adaptive in nature. Analysis of extracts of inoculated chicken skin by polyacrylamide-gel electrophoresis showed that the alpha, beta, and gamma components of the neutral-salt-soluble fraction of collagen were attacked.Keywords
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