HIV-1 Genome Dimerization: Formation Kinetics and Thermal Stability of Dimeric HIV-1Lai RNAs Are Not Improved by the 1−232 and 296−790 Regions Flanking the Kissing-Loop Domain

Publisher Website
Google Scholar
Abstract
The genome of all retroviruses consists of two identical RNAs noncovalently linked near their 5‘ end. Dimerization of genomic RNA is thought to modulate several steps in the retroviral life cycle, such as recombination, translation, and encapsidation. The kissing-loop model of HIV-1 genome dimerization posits that the 233−285 region of the HIV-1 genome, by forming a hairpin and initiating dimerization through a loop−loop interaction, is at least the core dimerization domain of HIV-1 RNA. This region is called the kissing-loop domain. In addition, it can be argued that sequences within the 296−401 region [Paillart et al. (1994) J. Biol. Chem. 269, 27486−27493] or 5‘ of the primer binding site [Laughrea & Jetté (1996) Biochemistry 35, 1589−1598] might play some role in the dimerization process. Accordingly, we have studied the effect of regions 1−232 and 296−790 on the dimerization kinetics and thermal stability of HIV-1Lai RNAs containing the kissing-loop domain (HIV-1Lai is a typical representative of North American and European HIV-1 viruses). Experiments conducted at high and low ionic strength indicate that these regions have no strongly positive effect on the dimerization process. Our experiments also indicate that the kissing-loop domain of HIV-1Lai has an apparent dissociation temperature 13 °C higher than that of the HIV-1Mal kissing-loop domain (HIV-1Mal is a Central African virus whose kissing-loop domain has a “weak” GUGCAC autocomplementary sequence). Because the 296−401 region of HIV-1Mal RNA stabilizes dimeric RNAs by ≤12 °C (Paillart et al., 1994), we infer that the contributions of sequences downstream of U295 are (at best) concealed in HIV-1Lai and in most American and European HIV-1 viruses, i.e., in viruses whose kissing-loop domain is characterized by a “strong” GCGCGC autocomplementary sequence.