Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution
- 12 June 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 366 (2-3) , 99-103
- https://doi.org/10.1016/0014-5793(95)00503-2
Abstract
We examined the complementation of various pairs of fragments derived from the streptococcal protein G B1 domain by NMR and CD. Most were not associated; however, one pair of fragments (1–40) and (41–56) interacted sufficiently enoughto regenerate a stable 1:1 complex, K d = 9 × 10−6M. A 2D-NMR analysis showed that the structure of the complex resembled that of native domain. Here we discuss the complementation from the viewpoint of the folding pathway of the protein.Keywords
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