Demonstration of the Ground Substance of Cartilage, Bone, and Teeth

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Abstract
A slab of bone about 5 mm. thick is decalcified in 5% HC1, washed, and placed for several days to 2-3 weeks in 3% KOH in 20% glycerin (if the bone is medium sized); for small bones the KOH should be decreased to 1%, and for large bones it may be increased to 5%. The soln. is changed frequently. When the bone begins to dissociate, it should be removed and washed in water till all traces of alkali are removed. The specimen is passed through 3 changes of dioxane into paraffin, then through a second paraffin bath into the final paraffin. Sections are cut at 10-12 microns and stained with VanGieson''s picro-fuchsin or with orcein.

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