Conformational peculiarities of rRNA inff supMet from E. coli as revealed by fluorescent methods
- 1 July 1976
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 2 (6) , 487-495
- https://doi.org/10.1007/bf00356938
Abstract
Conformational transitions in several individual tRNAs (tRNA inff supMet , tRNAPhe from E. coli, tRNA inf1 supVal , tRNASer, tRNAPhe from yeast) have been studied under various environmental conditions. The binding isotherms studies for dyes-tRNA complexes exhibited similarities in conformational states of all tRNAs investigated at low ionic strength (0.01 M NaCl). By contrast, at high ionic strength (0.4 M NaCl or 2×10-4 M Mg2+) a marked difference is found in structural features of tRNA inff supMet as compared with other tRNAs used. The tRNA inff supMet is the only tRNA species that does not reveal the strong type of complexes with ethidium bromide, acriflavine and acridine orange.Keywords
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