Calcium Inhibition of Pyrophosphatase in Crude Plant Extracts

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Abstract
Assays of alkaline pyrophosphatase activity in crude plant extracts are inhibited by soluble Ca coextracted with the enzyme from leaf tissues. Ca concentrations in most extracts are high enough to interfere seriously with the assay. All C4 plants [Zea mays L. Sorghum halepense (L.) Pers. Cynodon dactylon (L.) Pers. Setaria verticillata (L.) Beauv. Portulaca oleracea L. Atriplex halimus L. Atriplex tatarica L. Salsola soda L. Amaranthus lividus L. Amaranthus viridis L.] examined kept their soluble Ca and interference with pyrophosphatase activity at very low levels, whereas the C3 plants [Parietaria judaica Hal. Hedera helix L. Apium graveolens L. Helianthus annuus L. Viola tricolor L. Chenopodium opulifolium Schrader Arundo plinii Turra Stellaria media (L.) Vill. Capparis spinosa L.] covered the whole range of soluble Ca concentration. Low soluble Ca might be a prerequisite for high activity of pyrophosphatase and the C4 pathway.