Presence of a Prepiece at the NH2-TerminaI End of Pre-Cytochrome P-450(SCC) Peptide
- 1 May 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 95 (5) , 1525-1528
- https://doi.org/10.1093/oxfordjournals.jbchem.a134762
Abstract
Mild acid treatment of in vitro translated cytochrome P-450(SCC) (pre-P-450(SCQ) peptide cleaved the peptide into two fragments. Comparison of the sizes and the NH2-terminal amino acids of the fragments with those of the corresponding fragments from mature P-450(SCC) suggested that the prepiece of pre-P-450(SCC) was present at the NH2-terminal end of the peptide. This conclusion was confirmed by radio-sequencing of the NH2-terminal portion of pre-P-450(SCC).Keywords
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