The Development of Fundulus heteroclitus Embryos in Solutions of Metrazol

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Abstract
Certain aspects of gross behavior were studied in embryos of Fundulus heteroclitus which were immersed in metrazol solns. (0.1% or 2%) at different stages of development and which remained in them for varying periods of time. There was a marked effect on circulation in embryos treated between stages 23 and 32. Embryos treated between stages 23 and 26 exhibited localized trunk contractures; those treated between stages 23 and 32 were sometimes kyphotic or lordotic and sometimes showed abnormalities in mouth and opercular movements. Embryos treated later than stage 32 exhibited sharp lateral contractions and held their mouths wide open. Righting reflexes were lost during treatment in the latter group. Subjecting whole embryos to the action of metrazol provides a further method for studying the development of structure and function in the embryonic C. N. S.

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