IMMUNO-CYTOCHEMICAL LOCALIZATION OF TYPE-B COLLAGEN - COMPONENT OF BASEMENT-MEMBRANE IN HUMAN-LIVER
- 1 January 1980
- journal article
- research article
- Vol. 98 (3) , 591-+
Abstract
An indirect immunofluorescent method for localizing specific types of collagen was applied to unfixed cryostat sections of autopsy and biopsy specimens of normal and fibrotic human livers. Monospecific antibodies to Types I, III and B collagens were raised in goats by injecting collagens extracted from normal and fibrotic human livers. The antibody against B collagen stained the delicate sinusoidal meshwork within the lobule that closely paralleled in distribution the staining of reticulin fibers seen in classical silver preparations. Antibody to Type III collagen stained at the sinusoidal aspect of the hepatocytes in a pattern similar to anti-B antibody. All 3 antibodies tested strongly stained the portal tracts and the fibrotic bands in specimens with cirrhosis. Reticulin fibers within the liver lobule contain both Type III and Type B collagen.This publication has 17 references indexed in Scilit:
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