IMMUNOLOGICAL DETECTION OF CHINESE-HAMSTER OVARY CELLS EXPRESSING A MULTIDRUG RESISTANCE PHENOTYPE
- 1 January 1985
- journal article
- research article
- Vol. 45 (10) , 5064-5069
Abstract
A monoclonal antibody (IgG), has been prepared that specifically detects Chinese hamster ovary cells expressing a multidrug-resistant phenotype. This antibody recognizes the membrane P-glycoprotein (Mr 170,000) associated with drug resistance as determined by enzyme-linked immunosorbent assay with purified P-glycoprotein and by Western blot analysis of cell extracts from drug-resistant and drug-sensitive cells. By immunofluorescence methods, the antibody also reacts strongly with viable and ether:ethanol-fixed resistant cells but does not react with the parent drug-sensitive cell line. Thus, this antibody can bind with live cells allowing discrimination by immunohistochemistry between drug-resistant and drug-sensitive Chinese hamster ovary cells.This publication has 14 references indexed in Scilit:
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