Chitosan immobilization and permeabilization of culturedapium graveolens,chenopodium rubrum, anddaucus carotacells
- 1 January 1989
- journal article
- research article
- Published by Taylor & Francis in Food Biotechnology
- Vol. 3 (1) , 71-87
- https://doi.org/10.1080/08905438909549699
Abstract
Apium graveolens (celery) cells immobilized in coacervate capsules using alginate or carrageenan crosslinked with water‐soluble chitosan demonstrated cell outgrowth, respiration rates, and total protein recoveries equal to or greater than cells immobilized in the same polymers without the chitosan shell. Amaranthin recovery from cell extracts and medium of Chenopodium rubrum cell suspensions treated with dissolved water‐soluble chitosan were greater than pigment recoveries from acid soluble chitosan treated cells, and the cells demonstrated higher viability. Release from cell suspensions treated with 1,000 ug chitosan/ml medium was comparable to release from dimethylsulfoxide (DMSO)‐ treated suspensions. Protein release from Daucus carota suspensions treated with dissolved water‐soluble chitosan demonstrated increasing release with increasing chitosan concentration, maintaining viability greater than freely suspended controls even at 1,000 ug/ml levels.Keywords
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