Effects of in Vivo Administration of Pentagastrin, Secretin, and 13-Nle-Motilin on the in Vitro Incorporation of14C-Leucine into Protein of Human Gastric Mucosa

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Abstract
Under endoscopic control, biopsy specimens were taken from the oxyntic gland area of the stomach before and after administration of pentagastrin, synthetic secretin and 13-norleucine motilin (13-nle-motilin), respectively. In 29 volunteers, the basal rate of 14C-leucine incorporation into mucosal protein averaged 41.2 .+-. 7.7 .times. 103 cpm/mg protein (mean .+-. SD). One and 4 hours after s.c. administration of pentagastrin (6 .mu.g/kg body wt), values were significantly increased (P < 0.05) by 18.9 and 21.8%, respectively, with respect to the basal level. One hour after i.v. secretin, gastric mucosal protein synthesis was not substantially inhibited, whereas a 1 h continuous i.v. infusion of 13-nle-motilin (0.4 .mu.g/kg body wt per h) significantly decreased 14C-leucine incorporation rates by 17.5% (P < 0.05). In contrast to rats, 1 h after s.c. pentagastrin, protein synthesis in human duodenal mucosa was not altered. Pentagastrin has a trophic influence on gastric mucosa in man. The data are compatible with the hypothesis that gastrin and motilin may be involved in the regulation of human gastric mucosal protein synthesis.