Involvement of the exocyclic amino group of deoxyguanosine in DNA-catalysed carcinogen detoxification
- 1 January 1985
- journal article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 6 (1) , 147-149
- https://doi.org/10.1093/carcin/6.1.147
Abstract
The interactions of 7r,8t-dihydroxy-9t,10t-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE-I) with purified DNAs and synthetic polynudeotides of varying base composition have been studied. The ability of these polynudeotides to catalyse BPDE-I hydrolysis is strongly base-composition dependent, increasing with increasing (% G+C). Covalent binding of [3H](+)-BPDE-I to DNA shows a similar, linear dependence on base composition. Association constants for non-covalent binding exhibit no clear base-composition dependence. Poly(dI-dC) is relatively ineffective in catalysing hydrolysis, suggesting that the exocydic amino group of deoxyguanosine is involved in catalysis. Several plausible models for this dependence are presented, ranging from conformational changes with changing base composition to general add catalysis by the exocyclic amino group of deoxyguanosine.Keywords
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