Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings
- 1 January 1974
- journal article
- research article
- Published by CSIRO Publishing in Functional Plant Biology
- Vol. 1 (2) , 183-198
- https://doi.org/10.1071/pp9740183
Abstract
Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.Keywords
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