Different sensitivities of the murine melanomas BL-6 and BL-6-??m to local injections of interleukin-2 (IL-2). Analysis of gangliosides after the treatment

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Abstract
The murine melanoma cell line BL-6-βm, which Is a stable cell line transfected with a gene coding a unique actin subspecies called pm to the BL-6 cell line, has low metastatic potentials as compared with those of the parent cell line. BL-6-βm melanomas were found to be sensitive to In vivo local injection of IL-2, while BL-6 melanomas showed almost no response. Ganglloside analysis of BL-6 and BL-6-βm melanomas revealed that the main ganglioside of both melanomas was GM2, which suggested that different sensitivities between BL-6 and BL-6-βm melanomas to the Injection of IL-2 did not relate to the different compositions of main gangliosides. However, minor components of the gangliosides such as GM2 and GM1 emerged only In BL-6-βm melanomas after treatment with IL- 2. Local injection of IL-2 caused considerable Infiltration of antl-aslalo GM1-positlve cells Into the nests as well as the Interstttials of BL-6-βm melanomas. In contrast, In the BL-6 melanomas treated with IL-2, infiltration of the antl-aslalo GM1- posrtlve ceils was hardly seen, although anti-Thy1,2 and antimacrophage- posrtive cells were found to more or less the same extent as observed in BL-6-βm melanomas. These results suggest that the murine metastatlc variant melanoma cell lines BL-6 and BL-6-βm have different properties in terms of sensitivity to In vivo IL-2 treatment, and a slight enhancement of the ganglioside components GM2 and GM1 expression only in BL-6-βm after IL-2 treatment may play a role In the IL-2- mediated attraction of immune cells or may explain the different sensitivities of the two lines to treatment with IL-2.

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