Estimation of the Anion-Exchange Capacity of the Cell Wall ofNitella flexilis

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Abstract
Diffusion potentials in KCI and triphenyl phosphonium chloride (TPP) solutions have been measured across an isolated cell wall of Nitella flexilis either after pretreatment by difluoronitrobenzene (DFNB), a compound which reacts with the protein amino groups, or after alkalization of the external solutions. In both cases, it appears that the cationic transport numbers are enhanced with respect to the controls. These data suggest that the main effect of the two treatments was to deprive the wall of positive charges carried by protonized amino groups. The changes in tK were used to roughly estimate the anion-exchange capacity of the wall.

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