The distribution of subunit sizes of soluble proteins in human tissues

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Abstract
The distribution of subunit sizes of soluble proteins in human tissues was examined by gel filtration chromatography followed by SDS [sodium dodecyl sulfate] polyacrylamide gel electrophoresis. Up to 101 different polypeptides were identified. These appear to represent primarily the substituent polypeptides of easily solubilized non-enzyme proteins. The subunit sizes ranged between 7000-270,000 and the distribution was continuous, unimodal and skewed. The mean size was estimated at 54,600 .+-. 42,900. The distribution of subunit sizes and the overall mean were similar to those reported for easily solubilized human enzymes and other vertebrate proteins. The average structural gene coding such polypeptides probably consists of about 1400 base pairs.