Enzymatic Formation of Indole-3-Carboxaldehyde from Indole-3-Acetic Acid.

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Abstract
Indole-3-carboxaldehyde is a major reaction product of indoleacetic acid oxidase provided the system is coupled to the cytochrome-cytochrome oxidase system. Indole-3-glycolic acid yields indole-3-carboxaldehyde with equal efficiency both in the absence and presence of cytochrome c. Experiments with substituted indole-3-acetic acids as substrate indicate that the ring system, not the side chain, is the primary site of oxidation. The two position of the indole ring is probably the primary site of dehydrogenation. The use of indoleacetic acid-2-C14 as substrate made possible the detection of a reaction product which apparently is an aliphatic acid. The formation of this product is unaffected by the cytochrome system suggesting that it is the terminal oxidation product.