Cytotoxic t‐cell precursors against non‐immunogenic rat tumors: Limiting dilution analysis

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Abstract
After in vitro stimulation of lymphocytes with syngeneic tumor cells in the presence of interleukin 2 (IL‐2), a cytotoxic T‐cell response was observed against these spontaneously arising BDX tumors, which are non‐immunogenic in the syngeneic host. The response was due to cytotoxic T‐cells (CTL), which were specific for syngeneic tumor cells, i.e. neither syngeneic lymphoblasts, allogeneic lymphoblasts, allogeneic tumor cells, nor NK targets were lysed. The frequencies of anti‐tumor lymph‐node (LN) and spleen CTL precursors (CTLp) against a panel of syngeneic tumors of different histology varied between 1/6,000 and 1/16,000. Further analysis of the reactivity pattern of anti‐tumor CTL revealed some degree of cross‐reactivity within the syngeneic system. By a limiting dilution (LD) split culture approach in combination with cold target (CT) inhibition, it could be shown that tumors carry a panel of tumor‐associated antigens (TAAs). Some tumors express individually specific as well as cross‐reactive TAAs, while others carry cross‐reactive TAAs only.

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