Multi-step control of spindle pole body duplication by cyclin-dependent kinase
- 6 December 2000
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 3 (1) , 38-42
- https://doi.org/10.1038/35050543
Abstract
Organelles called centrosomes in metazoans or spindle pole bodies (SPBs) in yeast direct the assembly of a bipolar spindle that is essential for faithful segregation of chromosomes during mitosis. Abnormal accumulation of multiple centrosomes leads to genome instability, and has been observed in both tumour cells and cells with targeted mutations in tumour-suppressor genes. The defects that lead to centrosome amplification are not understood. We have recapitulated the multiple-centrosome phenotype in budding yeast by disrupting the activity of specific cyclin-dependent kinase (CDK) complexes. Our observations are reminiscent of mechanisms that govern DNA replication, and show that specific cyclin/CDK activities function both to promote SPB duplication and to prevent SPB reduplication.Keywords
This publication has 26 references indexed in Scilit:
- Mitotic Regulators Govern Progress through Steps in the Centrosome Duplication CycleThe Journal of cell biology, 1999
- Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin ANature Cell Biology, 1999
- Cyclin-dependent kinase 2 (Cdk2) is required for centrosome duplication in mammalian cellsCurrent Biology, 1999
- Centrosome hyperamplification in human cancer: chromosome instability induced by p53 mutation and/or Mdm2 overexpressionOncogene, 1999
- Cyclin-dependent kinase control of centrosome duplicationProceedings of the National Academy of Sciences, 1999
- Centrosome Amplification and a Defective G2–M Cell Cycle Checkpoint Induce Genetic Instability in BRCA1 Exon 11 Isoform–Deficient CellsMolecular Cell, 1999
- Requirement of Cdk2-Cyclin E Activity for Repeated Centrosome Reproduction in Xenopus Egg ExtractsScience, 1999
- Fluctuations in Cyclin E levels are required for multiple rounds of endocycle S phase in DrosophilaCurrent Biology, 1998
- Abnormal Centrosome Amplification in the Absence of p53Science, 1996
- S-phase-promoting cyclin-dependent kinases prevent re-replication by inhibiting the transition of replication origins to a pre-replicative statePublished by Elsevier ,1995