An Inductive Effector in Production of Extracellular Pullulanase byAerobacter aerogenes
- 1 October 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 46 (10) , 2425-2431
- https://doi.org/10.1080/00021369.1982.10865470
Abstract
The release of intracellular pullulanase (IP) of Aerobacter aerogenes was investigated to study the production mechanism of extracellular pullulanase (EP). A factor, named inductive effector (IE), which released IP from the cells was found in the culture broth of EP production. For IE formation, an initial medium pH of 9.0 was suitable, and the desirable culture period was 4 days. The IE fraction (MwA. aerogenes and released intracellular protease from the cells in shaking cultivation. Cell-bound pullulanase may be released by the proteases. Commercial endoproteases and lysozyme helped to make IP release, however, the mode of their action differed from that of IE.This publication has 3 references indexed in Scilit:
- Immunological Study of Pullulanase from Klebsiella Strains and the Occurrence of this Enzyme in the EnterobacteriaceaeInternational Journal of Systematic and Evolutionary Microbiology, 1979
- Pullulan, ein extracelluläres Glucan von Pullularia pullulansBiochimica et Biophysica Acta, 1959
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951