Production of the Second (C2) and Fourth (C4) Components of Guinea Pig Complement by Single Peritoneal Cells: Evidence That One Cell May Produce Both Components
Open Access
- 1 June 1972
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 108 (6) , 1609-1614
- https://doi.org/10.4049/jimmunol.108.6.1609
Abstract
Guinea pig peritoneal cells were examined by a hemolytic plaque assay for their ability to produce C4 and C2. Of cells obtained by peritoneal wash or from exudates induced by starch, 12% were capable of producing C2 and 12% were capable of producing C4. Peritoneal cells induced by oil or casein showed fewer cells capable of producing C4 or C2. With a plaque assay specifically designed to detect only those peritoneal cells that were producing both C4 and C2, we found one hemolytic plaque (produced by a single cell) per 1000 peritoneal cells. Cells capable of producing both C4 and C2 were identified as large mononuclear cells. The production of C4 and C2 by these cells was a temperature dependent process.Keywords
This publication has 0 references indexed in Scilit: