Short-Term Storage and Cryopreservation of Walleye Semen

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Abstract
The effects of additives, storage time, and temperature on cryopreservation of walleye (Stizostedion vitreum vitreum) semen were investigated. Semen was collected by aspiration, mixed with extenders containing ampicillin at either 180 or 3,325 μg/mL, and refrigerated for up to 14 d at 1 ° C. Egg fertilization trials resulted in mean fertilization values of 90.8% for the extender containing 180/μg ampicillin/mL, 86.4% for the extender with 3,325 μg ampicillin/mL, and 93.2% for fresh semen. Additionally, walleye semen was frozen with four different modified extenders containing dimethyl sulfoxide and other additives and thawed at either 32.1 or 21.1 ° C. Maximum egg fertilization rates were 83.2% for thawed semen and 96.6% for fresh semen. The initial results of this study indicate walleye semen can be effectively stored by cryopreservation but adjustments in storage techniques and extender ingredients may be necessary to improve results.

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