Role of the follicle cells and the oocytes in ecdysone biosynthesis and esterification in vitellogenic females ofLocusta migratoria

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Abstract
Follicle cells have been dissected from vitellogenic ovaries and isolated by collagenase treatment and centrifugation. The cell pellets were incubated with high specific activity tritiated 2-deoxyecdysone and 2,22,25-trideoxyecdysone. Both molecules were readily converted to ecdysone. A privileged sequence of hydroxylations was evidenced for the trideoxyecdysteroid: C-25; C-22; C-2. A significant amount of 2-deoxyecdysone and ecdysone was esterified to Helix hydrolysable conjugates both by the isolated follicle cells and by ooplasm preparations. In the ooplasm, the C-2 hydroxylase, but not the C-22 and C-25 hydroxylation systems, was found to be active. A relatively low level of C-20 hydroxylation was observed in the follicle cells.

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