INVIVO REGULATION OF MUSCARINIC CHOLINERGIC RECEPTORS IN EMBRYONIC CHICK BRAIN
- 1 January 1982
- journal article
- research article
- Vol. 21 (2) , 280-286
Abstract
Prolonged treatment of chick embryos in vivo with the muscarinic agonists oxotremorine or carbachol leads to dose- and time-dependent decreases in the number of brain muscarinic acetylcholine receptors (mAChR) as measured by the specific binding of the potent muscarinic ligand L-[3H]quinuclidinyl benzilate to brain membranes. Maximal doses of agonists reduced the number of mAChR as much as 44%. Maximal loss of mAChR occurs 4 h after treatment, but can be prevented or totally reversed within 24 h by blockade of agonist-receptor interactions with muscarinic antagonists. After sustained in vivo oxotremorine treatment, brain mAChR show a decreased apparent affinity for agonists owing to a decrease in the affinities of both the high- and low-affinity agonist binding sites.This publication has 15 references indexed in Scilit:
- Long-term regulation of muscarinic acetylcholine receptors on cultured nerve cellsLife Sciences, 1979
- Muscarinic acetylcholine receptor regulation by accelerated rate of receptor lossBiochemical and Biophysical Research Communications, 1979
- Cholinergic activity regulates muscarinic receptors in central nervous system cultures.Proceedings of the National Academy of Sciences, 1979
- Guanine nucleotides modulate muscarinic receptor binding in the heartBiochemical and Biophysical Research Communications, 1979
- Cardiac muscarinic cholinergic receptors. Biochemical identification and characterization.Journal of Biological Chemistry, 1978
- Regulation of adenylate cyclase activity mediated by muscarinic acetylcholine receptors.Proceedings of the National Academy of Sciences, 1978
- BINDING OF AGONISTS TO BRAIN MUSCARINIC RECEPTORS1978
- PERIPHERAL ACTIONS OF OXOTREMORINE, A METABOLITE OF TREMORINE1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- A series of normal stages in the development of the chick embryoJournal of Morphology, 1951