Cloning and sequence analysis of aflo/lfy homologue isolated from cauliflower (Brassica oleracea L. var.botrytis)
- 1 September 1993
- journal article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 22 (6) , 1163-1166
- https://doi.org/10.1007/bf00028986
Abstract
A cDNA library has been constructed from cauliflower curd in which floral development had been initiated. Two cDNAs (pBOFH3 and pBOFH8) have been isolated using theAntirrhinum flo gene as a heterologous probe. The two clones were sequenced and found to contain introns. Comparison of the deduced cDNA sequence ofbofh withflo and theArabidopsis homologuelfy reveals extensive homology. An mRNA transcript of 1.6 kb appears on northern RNA blots. This transcript can be detected, at low levels, before any obvious signs of floral differentiation, reflecting the rolebofh plays in determining floral meristem identity.Keywords
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