Location of protein S1 of Escherichia coli ribosomes at the 'A'-site of the codon binding site. Affinity labeling studies with a 3' -modified A-U-G analog
Open Access
- 1 July 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (7) , 1635-1646
- https://doi.org/10.1093/nar/3.7.1635
Abstract
An affinity analog with a 5-bromoacetamido uridine 5′-phosphate moiety bonded to the 3′ end of A-U-G has been prepared with the aid of polynucleotide phosphorylase. This 3′-modified, chemically reactive A-U-G analog was used to probe the ribosomal codon binding site. The yield of the reaction depended strongly on the ribosomal source and was sensitive to salt-washing ribosomes. The major crosslinking product was identified to be protein S1. Since the reaction of this 3′-modified A-U-G programmed ribosomes for binding, it is concluded that protein S1 is located at or near the 3′-side of the ribosomal codon binding site.Keywords
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