The 66 kDa component of eukaryotic initiation factor 3 interacts with globin mRNA and 18 S rRNA in preinitiation complexes
- 15 September 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 205 (2) , 171-174
- https://doi.org/10.1016/0014-5793(86)80891-2
Abstract
The 66 kDa protein present in a complexwith globin mRNA and 18 S rRNA [(1984) Eur. J. Biochem. 143, 27-33] has been reincorporated into functional eukaryotic initiation factor 3 (eIF-3) under conditions of protein synthesis. Additionally, two-dimensional polyacrylamide gel electrophoresis has been used to demonstrate the identity of the 66 kDa protein with the 66 kDa subunit ofeIF-3.Keywords
This publication has 18 references indexed in Scilit:
- Cross-linking of mRNA to initiation factor eIF-3, 24 kDa cap binding protein and ribosomal proteins SI, S3/3a, S6 and S11 within the 48S pre-initiation complexNucleic Acids Research, 1984
- Formation of a 22S mRNA . rRNA . protein complex during translation of globin messenger RNAEuropean Journal of Biochemistry, 1984
- Specific interaction of one subunit of eukaryotic initiation factor eIF-3 with 18S ribosomal RNA within the binary complex, eIF-3.small ribosomal subunit, as shown by cross-linking experimentsNucleic Acids Research, 1982
- Proximity of mRNA5′-region and 18S rRNA in eukaryotic initiation complexesNature, 1980
- A polypeptide in eukaryotic initiation factors that crosslinks specifically to the 5'-terminal cap in mRNA.Proceedings of the National Academy of Sciences, 1978
- Conservation of the primary structure at the 3′ end of 18S rRNA from eucaryotic cellsCell, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978
- Characterization of the ribosome-protected regions of 125I-labelled rabbit globin messenger RNAJournal of Molecular Biology, 1976
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970