Effects ofBacillus thuringiensisvar.israelensis20-kDa Protein on Production of theBti130-kDa Crystal Protein inEscherichia coli

1 January 1992

journal article
Published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry

Vol. 56 (9) , 1429-1433
https://doi.org/10.1271/bbb.56.1429

Abstract

A 20-kDa protein of Bacillus thuringiensis var. israelensis (Bti) has been shown to be necessary for the efficient expression of the 27-kDa mosquitocidal protein gene in Escherichia coli. We have investigated the effects of this 20-kDa protein on the expression of two 130-kDa mosquitocidal protein genes (cryIVA, cryIVB) in E. coli by supplying the 20-kDa protein gene in trans. When a recombinant plasmid, pLH4BX, which was constructed to express cryIVA under the E. coli lac promoter on pUC19, coexisted with the 20-kDa protein gene, a striking increase in production of the fused CryIVA was detected. This was not accompanied by an increase in the amount of intracellular mRNA, suggesting that 20-kDa protein exerts a posttranscriptional effect. We conclude that the 20-kDa protein also stimulates the production of 130-kDa protein in E. coli.

Keywords

ESCHERICHIA COLI

This publication has 0 references indexed in Scilit: