Polynucleotide Sequence Analysis by Sequential Base Elimination: 3′-Terminus of Phage Qβ RNA
- 21 November 1969
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 166 (3908) , 1004-1005
- https://doi.org/10.1126/science.166.3908.1004
Abstract
The nucleotide sequence of a 3'-terminal fragment obtained by ribonuclease T1 hydrolysis of the ribonucleic acid from bacteriophage Qβ has been determined by an improved method of sequence analysis which involves sequential removal of bases by periodate oxidation and beta-elimination. The results obtained from ten such oxidation-elimination cycles and from the alkaline hydrolysis of the remaining oligonucleotide indicate that the first 16 nucleotides at the 3'-terminus of this ribonucleic acid have the sequence: -G-C-C-C-U-C-U-C-U-C-C-U-C-C-C-A.Keywords
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