Structural Properties of the Heparan Sulfate Proteoglycans of Brain

Publisher Website
Google Scholar
Abstract
The heparan sulfate proteoglycans present in a deoxycholate extract of rat brain were purified by ion exchange chromatography, affinity chromatography on lipoprotein lipase agarose, and gel filtration. Heparitinase treatment of the heparan sulfate proteoglycan fraction (containing 86% heparan sulfate and 10% chondroitin sulfate) that was eluted from the lipoprotein lipase affinity column with 1 M NaCl led to the appearance of a major protein core with a molecular size of 55,000 daltons, as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. Comparison of the effects of heparinase and heparitinase treatment revealed that the heparan sulfate proteoglycans of brain contain a significant proportion of relatively short N‐sulfoglucosaminyl 6‐O‐sulfate [or N‐sulfoglucosaminyl](α1–4)iduronosyl 2‐0‐sulfate(α 1–4) repeating units and that the portions of the heparan sulfate chains in the vicinity of the carbohydrate‐protein linkage region are characterized by the presence of d‐glucuronic acid rather than l‐iduronic acid. After chondroitinase treatment of a proteoglycan fraction that contained 62% chondroitin sulfate and 21% heparan sulfate (eluted from lipoprotein lipase with 0.4 M NaCl), the charge and density of a portion of the heparan sulfate‐containing proteoglycans decreased significantly. These results indicate that a population of “hybrid” brain proteoglycans exists that contain both chondroitin sulfate and heparan sulfate chains covalently linked to a common protein core.