The relation of vitamin B1 to cocarboxylase

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Abstract
The synthesis of cocarboxylase from vitamin B1 has been shown to take place in the presence of washed dried yeast, hexosediphosphate and boiled tissue extract. The synthesis of cocarboxylase is almost completely inhibited by 0 [center dot] 005 M iodoacetic acid, but is only slightly inhibited by 0 [center dot] 04 M Na fluoride. Neither of the inhibitors affects the action of preformed cocarboxylase. By the proper combination of inhibitors, activators and enzyme, it is possible to differentiate between cocarboxylase and vit. B1 in boiled tissue extracts. The presence of both compounds has been demonstrated. The 2 compounds may exist in dynamic equilibrium in vivo. The possible mechanisms for the synthesis of cocarboxylase from vit. B1 are discussed in the light of the data presented.