Effects of Ozone on the Sporulation, Germination, and Pathogenicity of Botrytis cinerea

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Abstract
Studies were made to determine if B. cinerea conidia remain viable when grown in vivo and in vitro in the presence of ambient ozone levels and whether ozonized retain pathogenicity. Ozone at 599 .mu.g/m3 (0.30 ppm) for two 6 h periods significantly inhibited B. cinerea sporulation and germination in vivo and in vitro. Conidia ozonized in vitro at 299 .mu.g/m3 (0.15 ppm) for two 6 h periods caused less infection that non-ozonized conidia, based on total lesion area. No Botrytis lesions were induced on detached leaves [Pelargonium .times. hortorum] inoculated with ozonized conidia. Ozonization of conidia produced in vivo and in vitro decreased germination of conidia, germ tube length, pathogenicity and/or virulence.

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