Liquid Chromatographic Determination of Chloramphenicol in Calf Tissues: Studies of Stability in Muscle, Kidney, and Liver
- 1 May 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 74 (3) , 483-486
- https://doi.org/10.1093/jaoac/74.3.483
Abstract
A liquid chromatographic method has been developed for the measurement of chloramphenicol (CAP) in muscle, liver, and kidney. The mean recovery levels were 82.6, 75.3, and 79.2% in muscle, liver, and kidney, respectively. The method was repeatable and reproducible for CAP measurement in muscle, with a detection limit of 1 μg/kg. Investigation of CAP stability in muscle, liver, and kidney showed that CAP stability in muscle was good at -20°C; for spiked liver and kidney, degradation of CAP was observed, and the use of piperonyl butoxlde (PB) for metabolism Inhibition was recommended for recovery and linearity studies. However, PB was unnecessary for preservation of treated animal tissues if samples were cut into cubes and cooled at —20°C, Just after slaughter, pending analysis. With these limitations, CAP can be measured in liver and kidney.Keywords
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