Fluorescence Microscope Studies on the Development of Spindle Body and Chromosomal Fibers

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Abstract
Using diluteacrldine orange solution as a fluorochrome, the development process of the spindle body and the chromosomal fibers were investigated in vivo in pollen mother cells of Lilium longiflorum. Also, through such specific treatment secondary fluorescence was demonstrated. The transition of nuclear substances into atractoplasm, the nature of the chromosomal fibers and the fibrillar structure of the spindle body could be followed based on their fluorescent tints. The application of this technique to mitotic cells can elucidate the relationships between the morphological and chemical changes of cellular elements in the mitotic cycle.