Effect of Glucocorticoids on the Expression of γ-Glutamyltransferase and Tyrosine Aminotransferase in Serum-Free-Cultured Hepatocytes

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Abstract
Glucocorticoids exert a known beneficial effect on cultured [rat] hepatocytes when present in culture medium, maintaining their polygonal morphology and ultrastructural organization throughout the days of culture. Parallel to this excellent morphology, hepatocytes cultured in serum-free conditions, but with continuous presence of dexamethasone, retained after a week the ability to express tyrosine aminotransferase when stimulated by glucagon and glucocorticoids. The rise of .gamma.-glutamyltransferase was blocked in cultures supplemented by dexamethasone.