Plastocyanin as the Possible Site of Photosynthetic Electron Transport Inhibition by Glutaraldehyde

Publisher Website
Google Scholar
Abstract
Treatment of spinach chloroplasts with glutaraldehyde causes an inhibition in the electron transport chain between the 2 photosystems. Measurements of O2 flash yields, pH exchange and fluorescence induction show that the O2 evolving apparatus, photosystem II and its electron acceptor pool are not affected. The behavior of P700 indicates that its reduction but not its oxidation, is severely inhibited. Cytochrome f is still reducible by photosystem II but also slowly oxidizable by photosystem I. The sensitivity of isolated plastocyanin to glutaraldehyde further supports the conclusion that glutaraldehyde inhibits at the plastocyanin level and thereby induces a break between P700 and cytochrome f.

This publication has 19 references indexed in Scilit: