Nuclear-Magnetic-Resonance Studies of 5'-Ribonucleotide and 5'-Deoxyribonucleotide Conformations in Solution Using the Lanthanide Probe Method
- 1 July 1978
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 88 (1) , 259-266
- https://doi.org/10.1111/j.1432-1033.1978.tb12446.x
Abstract
The conformations of the metal-bound 5''-ribonucleotides and 5''-deoxyribonucleotides in aqueous solution at different pH values were studied using the lanthanide probe method. the conformational analysis, based on mixing different conformations in fast exchange within the NMR time scale, agrees well with the results from coupling constants, nuclear Overhauser effects and spin-lattice relaxation times, obtained for the metal-fixed systems. The equilibrium between the 2 basic conformational combinations for the 5''-nucleotides, anti-(N .dblarw. S)-gg-g''g'' and syn-(N .dblarw. S)-gt-g''g'' depends on the nature of the furanose ring, the base and also on the state of base protonation and phosphate ionization. The effect of base protonation is particularly strong for the guanine nucleotides.This publication has 29 references indexed in Scilit:
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