Comparison of direct wet mount and trichrome staining techniques for detecting Entamoeba species trophozoites in stools

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Abstract
Simulated freshly passed [human] stools containing motile E. moshkovskii trophozoites were examined by direct wet mount and permanent (trichrome) staining techniques. The percentage of detection by direct mount was 4.8%. The percentage of detection of the trophozoites by direct mount plus permanent stain was 58.5%. Laboratorians should be cautioned not to rely solely on the direct wet mount for detection or identification of protozoan trophozoites.