Analysis of Eukaryotic Translation in Purified and Semipurified Systems
Open Access
- 1 October 2000
- journal article
- unit
- Published by Wiley in Current Protocols in Cell Biology
- Vol. 8 (1) , 11.9.1-11.9.26
- https://doi.org/10.1002/0471143030.cb1109s08
Abstract
Much of the current understanding of the sequential steps involved in translation initiation has been obtained using sucrose gradients to isolate ribosomes and ribosomal subunits, as described here. These purified components are combined with purified translation factors to analyze the formation of intermediates in translation initiation and the roles of the translation factors in vitro.Keywords
This publication has 34 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- Ribosome Concentration Contributes to Discrimination against Poly(A)− mRNA during Translation Initiation in Saccharomyces cerevisiaePublished by Elsevier ,1997
- Efficient translation of poly(A)-deficient mRNAs in Saccharomyces cerevisiae.Genes & Development, 1994
- Translation factors as effectors of cell growth and tumorigenesisCurrent Opinion in Cell Biology, 1993
- TRANSLATIONAL CONTROL IN MAMMALIAN CELLSAnnual Review of Biochemistry, 1991
- Action of growth factors in the cell cycleBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1979
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- A simple general method to determine the proportion of active ribosomes in eukaryotic cellsExperimental Cell Research, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The effect of high ionic strength on monomers, polyribosomes, and puromycin-treated polyribosomesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970