Insertion/deletion-related polymorphisms in the human T cell receptor beta gene complex.
Open Access
- 1 October 1989
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 170 (4) , 1263-1270
- https://doi.org/10.1084/jem.170.4.1263
Abstract
Insertion/deletion related polymorphisms (IDRP) involving stretches of 15-30 kb within the human TCR-.beta. gene complex were revealed by pulse-field gel electrophoresis. Two independent IDRP systems were detected by analysis of Sfi I- and Sal I-digested human DNA samples using probes for TCR C and V region gene segments. The allelic nature of these systems was verified in family studies, and mapping data allowed localization of one area of insertion/deletion among the V gene segments and the other near the C region genes. All but one of 50 individuals tested could be typed for the two allelic systems, and gene frequencies for the two allelic forms were 0.37/0.61 and 0.46/0.54, indicating that these polymorphisms are widespread.Keywords
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