Detection by ELISA of immunoglobulin G subclass‐specific antibody responses in rotavirus infections in children

Publisher Website
Google Scholar
Abstract
IgG subclass‐specific antibody responses to a human subgroup 2 rotavirus were studied in 26 children by ELISA by use of monoclonal antibodies specific to the four human IgG subclasses. One hundred twenty‐nine serum samples were obtained before, during, and after an episode of rotavirus‐induced diarrhoea in these patients. When these sera were investigated, an increase in IgG1 and IgG3 subclass‐specific antibodies was detected in all 26 patients. IgG3 antibodies reached a peak concentration 1 week after rotavirus was detected in faecal samples and then progressively declined over the following months, whereas the peak concentration of IgG1 subclass antibodies was found 2 months later and seemed to persist thereafter. IgG2 rotavirus‐specific subclass antibodies were never found and IgG4 subclass antibodies were detected only in sera from seven of the 26 patients.