Comparison of Cell-Surface Human Melanoma-Associated Antigens Identified by Rabbit and Murine Antibodies
- 1 January 1982
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 1 (4) , 465-472
- https://doi.org/10.1089/hyb.1.1982.1.465
Abstract
Melanoma-associated antigens (MAAs) recognized by murine monoclonal and rabbit polycional antibodies were compared. Two rabbit anlimelanoma sera raised in our laboratory recognized five human cell-surface MAAs with approximate MWs of 75, 95, 120, 150, and 240 kd. These antigens were easily detected by SDS-PAGE of specific immunoprecipitates on a melanoma cell (HM31) which failed to reveal antigens reactive with the panel of murine monoclonal antibodies studied. By contrast, these five antigens could not be detected on another melanoma cell (SK-MEL-28), which was reactive with several of the murine monoclonals. These results suggest that the MAAs recognized by rabbit and murine antibodies are different and imply that the antigens which are immunogeneic in man may not necessarily be immunogeneic in mice.Keywords
This publication has 1 reference indexed in Scilit:
- Identification and solubilization of iodinated cell surface human melanoma associated antigensInternational Journal of Cancer, 1977