Isotachophoresis in Free-Flow Using a Miniaturized Device
- 26 April 2006
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (11) , 3815-3819
- https://doi.org/10.1021/ac060063l
Abstract
For the first time, we report a miniaturized approach for isotachophoresis employing the technique of free-flow electrophoresis. Using a micromachined separation chamber with a volume of 200 nL, a sample mixture of fluorescein, eosin G, and acetylsalicylic acid was separated, stacked, and concentrated in less than a minute. Additionally, an isotachophoretic separation of a reaction mixture of myoglobin and fluoresceinisothiocyanate as a fluorescence label has shown the potential of this method for on-line sample preparation.Keywords
This publication has 6 references indexed in Scilit:
- Free-flow zone electrophoresis and isoelectric focusing using a microfabricated glass device with ion permeable membranesLab on a Chip, 2006
- Free-Flow Electrophoresis on an Anodic Bonded Glass MicrochipAnalytical Chemistry, 2005
- Free-flow electrophoresis in a microfabricated chamber with a micromodule fraction separatorJournal of Chromatography A, 2003
- Free Flow Electrophoresis Device for Continuous On-Line Separation in Analytical Systems. An Application in Biochemical DetectionAnalytical Chemistry, 2000
- Separation of plant membranes by electromigration techniquesJournal of Chromatography B: Biomedical Sciences and Applications, 1999
- Fabrication of low loss polymer waveguides using injection moulding technologyElectronics Letters, 1993