Interaction of Oxidative Stress and Inflammatory Response in Coronary Plaque Instability
- 1 August 2003
- journal article
- research article
- Published by Wolters Kluwer Health in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 23 (8) , 1398-1404
- https://doi.org/10.1161/01.atv.0000081637.36475.bc
Abstract
Objective— C-reactive protein (CRP), a predictor of cardiovascular events, localizes in atherosclerotic arteries and exerts proinflammatory effects on vascular cells. Reactive oxygen species (ROS) have been implicated in atherogenesis and plaque instability. Methods and Results— Expressional pattern of CRP in directional coronary atherectomy specimens from 39 patients was examined. Characteristics of histological plaque instability and higher levels of serum CRP and fibrinogen were associated with the CRP immunoreactivity. In situ hybridization revealed the presence of CRP mRNA in coronary vasculature. Furthermore, the expression of CRP mRNA and protein was detected in cultured human coronary artery smooth muscle cells (CASMCs) by reverse transcriptase–polymerase chain reaction and Western blotting. In addition, CRP was frequently colocalized with p22phox, an essential component of NADH/NADPH oxidase, which is an important source of ROS in vasculature. Moreover, the incubation of cultured CASMCs with CRP ...Keywords
This publication has 27 references indexed in Scilit:
- Loss of Pentameric Symmetry of C-reactive Protein Is Associated with Delayed Apoptosis of Human NeutrophilsJournal of Biological Chemistry, 2002
- Mechanisms of Increased Vascular Superoxide Production in Human Diabetes MellitusCirculation, 2002
- Generation of C-Reactive Protein and Complement Components in Atherosclerotic PlaquesPublished by Elsevier ,2001
- Angiotensin II-mediated hypertension in the rat increases vascular superoxide production via membrane NADH/NADPH oxidase activation. Contribution to alterations of vasomotor tone.Journal of Clinical Investigation, 1996
- Increased expression of matrix metalloproteinases and matrix degrading activity in vulnerable regions of human atherosclerotic plaques.Journal of Clinical Investigation, 1994
- Production of hydrogen peroxide by transforming growth factor-beta 1 and its involvement in induction of egr-1 in mouse osteoblastic cells.The Journal of cell biology, 1994
- Oxygen radicals as second messengers for expression of the monocyte chemoattractant protein, JE/MCP-1, and the monocyte colony-stimulating factor, CSF-1, in response to tumor necrosis factor-alpha and immunoglobulin G. Evidence for involvement of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent oxidase.Journal of Clinical Investigation, 1993
- Hypercholesterolemia increases endothelial superoxide anion production.Journal of Clinical Investigation, 1993
- Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1Nature, 1991
- HAEMOSTATIC FUNCTION AND ISCHAEMIC HEART DISEASE: PRINCIPAL RESULTS OF THE NORTHWICK PARK HEART STUDYThe Lancet, 1986